The association of the paracetamol and the diclofenac (PARA+DiCF) is the aim of our study. 60 male rats "Albinos wistar" were treated by oral gavage (per os) during seven days. A control group was treated by mineral water (0+0) mg/kg and a second group was treated with only a toxic dose of 100 mg/kg of PARA (100+0) mg/kg. Remaining lots were treated with a combination of different toxic doses of PARA and a therapeutic dose of DiCF (15+3, 100+3, 200+3 and 400+3) mg/kg. Plasma concentration of amiotransferases (ASAT, ALAT), alkalines phosphatases (ALP), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione (GSH), glucose, cholesterol, creatinin, direct and total bilirubin, significantly varied in the treated rats regarding to the witness's rats. The toxicity of PARA reveled by a dose dependant augmentation in the blood of ASAT, ALAT, ALP, GPx, GR, glucose, creatinin, bilirubin, and by decrease serum cholesterol concentration and tissue GSH comparing to controls. The results of the depletion of GSH and the augmentation of the oxidative stress enzymes (GPx and GR) suggest a detoxification function of the system of glutathione. The association (PARA+DiCF) revealed a protector effect resulting in the reduction of the concentrations of ASAT, ALAT, ALP, GPx, GR, bilirubin with the increase of GSH. Regarding to all these results, it has been suggested that DiCF has a protective action towards the toxic effects of PARA.
Keywords: Paracetamol, diclofenac, oxidative stress, toxicity, glutathione and glutathione peroxidase.
